Check the solvent line filters for permeability. Passivation is a metal finishing process to prevent corrosion and resist rust. Pump problem – Contact your maintenance provider. Agilent recommends a purge of at least 30mL in total for the 1200 systems. Pump is compatible with HPLC and UHPLC, handling 2. Use only HPLC-grade solvents and water filtered through 0. The source of column clogging comes from particulates in a number of places:We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. thermoscientific Vanquish Pump H VH-P10-A-02 Operating Manual 4820. I usually let the column drip into a beaker while washing it. 9-0. . This pump operates at pressures up to 600 bar and flow rates up to 10 mL/min. The 1260 Infinity II Flexible Pump is a quaternary UHPLC pump that provides gradient formation and solvent blending from up to four solvents. 9 Pump Types LPG pump type Eluent composition at the low pressure side => Before the pump head Eluent is composed through proportioning valves Eluent segments pass working and equilibration cylinder Up to four solvents can be mixed HPG pump type Eluent composition at the high pressure side => Behind the pump head Pure solvents pass. Purge pump at high flow rate (e. 1:55. It is modified from of gas chromatography, it is applicable for both Volatile as well as Non volatile compound. Run through the prime purge valve first (10 mls), then. Thus, the gradient delay volume of an HPLC system is the volume that the pump must deliver until a change in solvent composition reaches the column inlet. e. Rugged and dependable, Arc HPLC System delivers the ideal balance of performance and value for right-the-first-time analysis. Prepare the Pump • Use fresh or different mobile phase (as required). 18: Ready Check Results window appears after the flow meter purge function is If the conclusions from the article How to Troubleshoot no flow or no pressure issues: Binary/Quaternary/Isocratic Pumps - G131XX, and G711XX, and performing the Pump Leak Rate Test, points to a defective or clogged AIV, you may try the following procedure: How to Sonicate the Inlet and Outlet Valves of Agilent 1100, 1200 and 1260 Series Pumps. First, disconnect connection (1) in Figure 1 and pump the solvent (to check the backpressure tube 4)). However, in order to obtain a full understanding of the pump, Thermo Fisher Scientific recommends that you review the manual thoroughly before beginning operation. Dolan advises that this will clear. Mobile phase is passed through porous polymer tubing placed in a vacuum chamber. Discover the efficient LC-40D pump for routine HPLC applications up to 44 MPa (440 bar. . Prime the pump with degassed solvent. If the solvent line is "dry," perform "dry" primes by opening the manual prime and purge valve, and draw out 10 mL from each solvent line using a syringe. Prime the HPLC pumps under the following conditions: - starting the pumps for the first time after it has been idle for more than 24 hours. 8. Before using an HPLC system, you should prime all of the lines in your HPLC pump. 19 Section 3: Pump Maintenance 20 Purge Valve 21 Outlet Ball Valve 21 Active Inlet Valve (AIV) 22 Seals and Pistons 25 Pump Head 26 Parts and Supplies 27 Solvent Inlet FiltersChromaster HPLC 5110 pump with auto-purge valve. Consumables likely need to be replaced on aqueous pump. L/mni for 5mni . Overall Routine Pump Maintenance 1. Figure 2 shows how to perform the purge for an i-Series. 1 Introduction Purpose of the User Manual The User Manual provides you with instructions and information to run the ÄKTA™ avant system. Flow anomalies can interfere with the chromatography and stand in the way of repeatable results. HPLC pumps can be single, binary, or ternary, meaning that the can make use of one, two, or more different solvent reservoirs. Fig 2. Autosampler – draws samples from vials and injects them into the solvent flow provided by the pump. Program the pump to purge the column with strong solvents. Pump 15 min at this pressure to wear the seals in. Seal could have fluoropolymer O-ring energizer instead (black O-ring). Reciprocating pump, syringe pump, and pneumatic pump. 2. Monitoring your HPLC system for potential issues can help to. If [Auto Purge] is not set in the method use the manual purge option by opening the drain valve and pressing the [Purge] button on the instrument. The system pressure and PreColumn pressure also fluctuates between 0. Purge valve Purge functionFig. Figure 2 shows how to perform the purge for an i-Series. However, in order to obtain a full understanding of the pump, Dionex recommends that you review the manual thoroughly before beginning operation. 5. Mass spectrometry, chromatography, spectroscopy, software, dissolution, sample handling and vacuum technologies coursesRemedy. When they fill with air, they lose their prime and stop pumping. off. Column frits are a common point of clogging. First statement: Don’t let it run dry. - after changing solvents. Meet Your New Lab Ally - the Alliance iS HPLC System. A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head. Treatment 1: Open a purge valve. categories. Delivering Scientific Insights to Improve Human Health and Well-Being. One option is to apply vacuum during filtration of mobile phase through 0. The 1260 Infinity II Flexible Pump combines high performance and simplified operation with highest flexibility in automated gradient formation and solvent blending. Use a syringe to draw enough solvent for completely filling the tubings to the pump inlet before continuing to prime with the pump. You can purge all channels using a composition of 25 % for each channel, using warm water in all four channels. The EVAP system is designed to prevent fuel vapors from escaping into the atmosphere by capturing and temporarily storing them in a. Treatment 1: Open a purge valve. Use solvent inlet filters to protect the system from incoming particles. Change the gradient. This will redirect the solvent flow to the waste exit of the valve instead of passing through the whole system. *Run to run RT variation of ±0. HPLC maintenance tutorial on how to change the High Pressure Filter on an Agilent. Preparation steps: Flush the pump head with an appropriate solvent using the purge function. Then wash them out with water and re-assemble them on the pump head. Read Less. Open the purge valve on the Agilent Infinity 1260 isocratic pump b. ID stainless steel tubing kit • Galaxie Chromatography Software-0. This video shows the operation a HPLC purge valve. Run at high flow: Next, Dolan advises to run the pump for several minutes at high flow (e. Priming and Purging the 1525 Binary HPLC Pump. The system must be primed to remove air bubbles and for the pump to function properly. So, let's get started and purge away any confusion! Purging, in the context of lab equipment, is the process of flushing a lot of. Hello Kancheri this is the purge valve for Agilent 1260 G1312-60071 | Agilent You can check in the manual at page 154 on HOW to replace purge valve with this. 1. Replace the filter frits if necessary. 02 min SD (whichever is larger) of the set flow rate. Maintaining the Purge Valve 139 Maintaining the Pump Head Assembly 140 Procedure 1: Pump Head with old Plunger Housing 141 Stage 1: Removing the Pump Head Assembly 141. UltiMate 3000 Series: SD, RS, BM, and BX Pumps Page II Operating Instructions. 5301 Stevens Creek Blvd. ) If system has check valve, loosen valve to allow air to escape. 결과 및 정리. The ultra-high pressure capacity (up to 130 MPa/1300 bar) allows the of sub-2μm particle columns for high efficiency chromatography, especially for LC. Move to step 2. Insert the suction tube for the flow channel to be purged into the reservoir bottle containing the mobile phase and place the bottle on the reservoir tray. This will prevent any flushed contaminants from getting lodged into the latter components of the system. Prepare the 20 ppm of caffeine in HPLC water by diluting 2 ml of stock solution to 50 ml with HPLC water and inject the triplicates of 5 µl, 10 µl, 20 µl, 50 µl and 100 µl of sample preparation. Open the black purge valve on the front of the quaternary pump by turning it several times counterclockwise. Unit conversion calculator for converting commonly used unitsSJ: There are two diffusion paths involved in inline degassing (ignoring vacuum side diffusion). Vanquish Binary Pump C: Up to 700 bar (10,000 psi) and 10 mL/min. 1 uL/min to 10 mL/min with accuracy of ± 1 % or ± 2 µL/min (whichever is larger), and precision < 0. See Reference 1 for interactive video. 5 mL/min c. 2 Push the “conc” button on LC20AD. ( refer to Pump Operating Manual ). Consumables likely need to be replaced on aqueous pump. They are available in various configurations (→ Supported Configurations, page 5) and can thus be used in numerous laboratory environments. • Purge each channel for at least 10 min at 5 mL/min. OBJECTIVE: To clean and flush an Alliance HPLC System or an ACQUITY UPLC system to remove contaminants. Among the various technologies developed for chromatography,. The following diagrams illustrate the operating principle of a modern HPLC pump: an electric motor drives a piston (generally made of sapphire or ceramic material) in and out of metal, ceramic, or PEEK piston chamber. Make sure that the AVR7-3 inject valve is in the Purge position. Purge the system to remove air bubbles and make sure the solvent is the same solvent when the GPC column is placed. 1ml/min2 g. HPLC Pump – provides solvent flow and proportioning. ), prime system if necessary. For modular autosamplers purge from front panel. 17: Ready Check Results window appear s after the NC pump blocks purge is executed 2. 3 Page 5 Column Observations Potential Problems[][]Purge the pump with fresh diluent (filtered and degassed). 2. This sets the mobile phase composition to 100% water so we can purge the water line. Channels in column Replace column. Set a flow of 5 mL/min and purge for 5 min. 2 Wellplate Autosampler Purge Kit Features Features The new Wellplate Autosampler Purge Kit offers the following features: • Fits for Agilent 1100 Series Wellplate Autosampler (G1367A and G1368A (thermostatted)). Open prime/purge valve (in the case of Chrom Tech pumps, you turn the knob counterclockwise one to two turns. Worldwide Emails . This will redirect the solvent flow to the waste exit of the valve instead of passing through the. Successful HPLC Operation Part of Thermo Fisher Scientific chromatography. Replace the O-Ring on the Purge Valve 150 Remove the Pump Head Assembly 153 Maintenance of a Pump Head Without Seal Wash Option 155HPLC troubleshooting guide Introduction During the development of a new HPLC method, there were unexpected results—all peaks, except for the. Pump – Open the purge valve and purge every solvent channel with 5 mL/min for 5 minutes to remove bubbles in your solvent lines (Note: if the backpressure at purging is higher than 10 bar, please replace the PTFE frit in the purge valve) – Make sure that the flushing solvent is mixable with the storage solvent of your solvent channel. 3601-EN-5. b. Leaking pump heads Check the seals and replace, if necessary. 6 x 300 mm, 3 µm, 150 Å). The FRC-40 features an open-access design, a parameter simulation to ensure pure fractions, compatibility with many racks, and up to 4-channel acquisition. 0Mb)Page 35: Purging The Pump The pump should never be used for priming/purging empty tubings (never let the pump N O T E run dry). 3. Purge pump at high flow rate (e. Select this procedure when you want to prime and purge any 1525 Binary Series Pump. Make sure that you know which solvent is linked to which pump. This technique is commonly used in labs for the purpose of separating, identifying, and quantifying components in a mixture. Purge valve open. Count on the Arc HPLC System for high-efficiency separations and quality data to help meet your regulatory requirements with confidence. The pump offers a dynamic flow rate range from 1 to 50 mL/min at up to 420 bar, allowing workflows on. Leak at pump 18 Selectivity Differences in selectivity 19 Baseline Disturbance at void time 20. Search. Pump heads with maximum flow rates of 10 and 50 ml/min are available. DO NOT PURGE THE SYSTEM WITH THE BALL VALVE CLOSED! THIS WILL RESULT IN PRESSURE TOO HIGH FOR THE PUMP TO HANDLE AND CAN RUIN THE COLUMN!!! 6. 6 Purging the Pump. Retention. HPLC pumps incorporate single or dual piston, syringe, or diaphragm pump designs. This pump enables quaternary solvent delivery at pressures up to 800. Reassemble pump head and reinstall. If your problem is. If problem persist try regenerating the column using directions given by the manufacturer, such running methanol for 10 minutes, followed by hexane, ethanol and methanol again. Vacuum degassing: the solvent is exposed to a vacuum and the reduced pressure removes more than 60% of the dissolved air. Priming and Purging the 1525 Binary HPLC Pump. ResearchGate | Find and share researchpump for extended seal lifetime † Setting upper and lower pressure limits will turn off the pump, e. pump에 대한 자세한 설명은 아래 링크를 참고해주세요! ANSWER. g. From my little experience i learnt that it may be due to air trapped in. Priming and Purging the 1525 Binary HPLC Pump. For more information on purging the pump, see the reference. , 5-10 mL/min. 6. The manual is in English and can be downloaded as a PDF file. Up to 6 FRC-40’s can be configured. Flush the pump with 100% filtered, distilled water for at least 3 minutes. The pump inlet port is closed to allow the pump to run with the gas ballast valve in the open position, which helps to purge the pump. 9 Purge a short while longer, and then close the purge valve. 1) Locate the pump draw-off valce (handle located on front of the pump) and priming syringe. You. After waiting a specified time, it starts pumping the mobile phase at the flow rate specified in the analytical method to automatically equilibrate the. Disconnect tubing at guard column (if present) or analytical column inlet. View more in. Figure 1 Overview of the binary pump Purge valve Active inlet valve Mixer Outlet valve. Related: 6. Learn essential HPLC maintenance tips. You may find that the manufacturer of your particular. Contacting Us Vanquish Pumps (VH-P10) Operating Manual Page 3 Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales support for HPLC products, contactSolving Problems That Matter - Batteries. g. Honoring the Past, Focusing on the Future: A Conversation with Jess Lee. HPLC에서의 purge는 불필요한 기체를 제거하기 위해, 2. This video shows the operation a HPLC purge valve. Purge Valve Assembly: 1 - Valve body, 2 - PTFE frit with groove, 3 - Seal cap. degasser ports and/or pump head multichannel valve or pump head inlet, as needed, to draw liquid through until all lines are filled. 2 Push the “conc” button on LC20AD. 0 ml/min flow for more than 2. Reducing the diffusion path to improve degassing. For best performance you must know your eluents 2. On the front panel of the pumps, press the VP button. Make sure to confirm that the purge valve isHow to prevent your HPLC from running dry. Delivering Scientific Insights to Improve Human Health and Well-Being. Treatment 2:Open the compression fitting at the top of the outlet check valve with a slow pump flow until solvent leaks around the fitting. 8 Click pump on the flow diagram and change the flow path into A2 and B2. Power off the pump and remove the waste tubing from the purge valve. Flush the pump with 100% filtered, distilled water for at least 3 minutes. Open the purge valve and purge the system for 5 min with isopropanol at a flow rate of 2 mL/min. 6. Put the Pump A in your buffer. HPLC maintenance tutorial on how to remove the pump head on an 1100/1200/1260 Agilent HPLC. • Mast kit with mixer, purge valves and manual injector • Pre-formed 0. 18: Ready Check Results window appears after the flow meter purge. High Pressure Liquid Chromatography. 23 MPa. Second, the dissolved gasses must diffuse through the membrane (flat film or tubing wall). Prepare the solvent: Begin by ensuring that the solvent is filtered and degassed. Select this procedure when you want to prime and purge any 1525 Binary Series Pump. 1–1% solution by volume is sufficient. 000, Ready, 0. Popular answers (1) There can be several reasons for the pressure drops you observe: air could still be present in the system after your recent repair (i. Changing the Purge Valve Frit/Purge Valve for Binary Pumps Headquarters | Other sites. 05. The system will start the countdown from 5 minutes to zero. (Most ternary pumps actually have four solvent reservoirs. For reverse-phase columns, wet the column using a mobile phase like methanol and bring it to normal flow rate. The 1290 Infinity II Flexible Pump combines highest performance, methods compatibility, and. 02 in. Increase flow to 5 mL/min 2. Relevant functions marked for purging, flow settings ect. Open the Purge Valve of your pump and purge each solvent line, one by one, with HPLC-quality Isopropanol (IPA). Yes it looks like this! Now the valve is ok and there’s no more leak, maybe now the frit is soaked by the mobile phase and fits better?! Thanks!! reply from dmarchini< dmarchini >…. Draw approximately 20 mL of fluid. See "Pressure pulsation or inconstant pressure" in Pressure on page 120. 1290 Infinity II High Speed Pump. (Prime each pump head separately. • Vacuum chambers – Contain the gas-permeable eluent channel. So for example, if you normally run at 100 bar and the pressure drops to 5 bar, then the pump will shut down. Achieve binary-like performance in your HPLC instrument. 6:51. Pump Injector/Autosampler Column Detector Data System/Integrator HPLC System Components Problems Can Be Related to All Components in the System Page 4 Categories of Column and System Problems A. HPLC Pump: The HPLC pump produces high pressure that gives a continuous and reproducible flow to the mobile phase throughout the HPLC system. Rough pumps stay on so the system remains under vacuum. TOC 측정기에서의 purge는 제거가능한 유기탄소를 제거하기 위해, 3. 5 Close the purge valve, set the flow to a rate adequate to achieve a pressure of 350 bar. Filter all samples and standards 3. This video shows the operation a HPLC purge valve. Wash the system with isopropanol (excluding the column). This pump operates at pressures up to 600 bar and flow rates up to 10 mL/min. It also enables quaternary solvent delivery at. 7:30. g. From the data analysis program vent the system. 4411-EN Revision 2. General Recommendations For effi cient reduction of carryover, it is important that the sample be soluble in weak and strong wash solvents. 5 mL/min d. Between samples when your compound is very sticky (apolar) to help clean the column. HPLC is an abbreviation for High Performance Liquid Chromatography. ISET enables. Blocked channels can often be released by purging with a high flow with warm water (60 to 70 °C, HPLC grade). PROCEDURE: Flush the system using one of the cleaning mixtures as described in the cleaning. The degasser is a standard feature of the 1525µ Binary HPLC Pump and an optional feature of the 1525 analytical and 1525 EF Binary HPLC Pumps. 5 01. Initially, the pressure was unstable. See your pump modules user manual for detailed instructions on re-assembly. BEFORE WORKING WITH THIS ANALYTICAL HPLC 1. Use only HPLC-grade solvents and water filtered through 0. We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. Two of the most widely used methods to degas a mobile phase are: Helium purging: helium is bubbled through the solvent and removes up to 80% of dissolved air. L/mni for 5mni . Initial investigation should consist of purging the pump with fresh eluent to confirm no air is present within the pump, as the presence of air within the pump may cause fluctuations in pressure. Leak check: the Vice President HPLC, Fraser McLeod. 45 µm inert hydrophilic filter (avoid cellulose) and degas all eluents thoroughly, using sonication under vacuum. 1290 Infinity II High Speed Pump User Manual. • Use less-vigorous purging • Prepare fresh mobile phase • Check pump. Change the pump program back to 50/50 water/ACN to re-equilibrate the system e. Certificates. For more information, visit tutorial demonstrates how to prime the pumps in Bio-Rad's new NGC liquid chromatography sy. 1260 Infinity Binary Pump User Manual 11 Introduction to the Binary Pump 1 Instrument and OperationThe pump assembly includes a pump head with a passive inlet valve and an outlet valve. The user manual for the Agilent G1311A Quaternary Pump, a high-performance liquid chromatography (HPLC) module that provides reliable gradient formation and precise flow rates. . We have a HPLC Agilent 1100 with pressure problems. If the pressure reaches its maximum (press. 2. Mass spectrometry, chromatography, spectroscopy, software, dissolution, sample handling and vacuum technologies courses. 5. Purge valve Purge function Remove the purge valve waste line and the capillary from the purge valve outlet. After waiting a specified time, it starts pumping the mobile phase at the flow rate specified in the analytical method to automatically equilibrate the. Purge Valve Assembly: 1 - Valve body, 2 - PTFE frit with groove, 3 - Seal cap. The Waters 1500 Series HPLC Pump Installation and Maintenance Guide has five chapters and four appendixes. Repeat twice after the cycle has finished after approximately 4 min. Priming and Purging the 1525 Binary HPLC Pump. Replacing a Check Valve Cartridge. Peaks Peak area precision Pressure Retention times Baseline Troubleshooting overview Running into issues with your HPLC or UHPLC analysis?8th May 2023 HPLC Pump Care and Troubleshooting Many of the technical support inquiries we receive at Chrom Tech are related to care and troubleshooting of our. OBJECTIVE: To clean and flush an Alliance HPLC System or an ACQUITY UPLC system to remove contaminants. Purge each mobile phase that will be used by selecting “Purge” on the PumpModule tab on Chromeleon. Check for flow. Using a solvent filtration kit or an online degasser will remove any dissolved. g. Replace the O-Ring on the Purge Valve 120 Remove the Pump Head Assembly 122 Maintenance of a Pump Head without Seal Wash 124 Maintenance of a Pump Head with Seal Wash 127• Vacuum pump – An electrical two-speed stepper motor pump that creates a vacuum in the vacuum chambers. Left pump connected to Vector PC post column derivatization. 2 to 0. Each channel comprises a pump assembly including pump drive, pump head, active inlet valve with replaceable cartridge, and outlet valve. The two channels are fed into a low-volume mixing chamber which is connected via a restriction capillary coil to a damping unit and a mixer. We routinely see HPLC systems with white fluffy crystals built up around the pump heads, pistons and various fittings from lack of maintenance on a daily basis. 88 Service Handbook for 1050 Series of HPLC Modules - 11/2001 Pumps: General Information Specifications Specifications Table 2 Specifications of 1050 PumpsHi,when I do wet prime/dry prime in hplc ? Thank you. Cap seal for purge valve knob; Cleaning swabs; Figures. If problem persists, flush system with 100% methanol or isopropanol. Purging air from pump system: Change solvent line to a bottle containing 100% isopropanol. 5 ml/min for 4 min, I only collected 1 ml of liquid. Feeding the degasser with an inferior mobile phase will create inferior results. Solving Problems That Matter - Bioprocessing. close the purge. HPLC에서의 purge는 불필요한 기체를 제거하기 위해, 2. Prime the pump with degassed solvent. Vanquish Binary Pump N: Up to 1500 bar (22,000 psi) and 100 µL/min with active flow control. 1, 3, and 4. Set the flow rate to 5 ml/min d. 5 um columns, use 0. "Chromatography" is a technique for separation, "chromatogram" is the result of chromatography, and "chromatograph" is the instrument used to conduct chromatography. Parallel dual micro plunger (10 µL) construction for low pulsation and stable baseline. After the valve sonication procedure, it is suggested to purge the pump with water at 60 degrees, keeping the purge valve open. closed. Focus on the column's inlet: After the initial flush, reduce the flow rate to 1 mL/min or so and close the purge valve until no more bubbles emerge. Two of the most widely used methods to degas a mobile phase are: Helium purging: helium is bubbled through the solvent and removes up to 80% of dissolved air. Is the pump fuse in working order?Discover faster analysis, better resolution, and more reproducible chromatography with our new flagship UHPLC pump. Maintaining your LC columns. 0046. 02 in. Remove and clean pistons. A peristaltic pump (piezo pump) delivers fresh wash solvents to the fl ush port. I am working with HPLC Shimadzu 20AD fitted with LC20AD. Problem 3:Inconsistent or too slow pump flow rate. In a low-pressure gradient (LPG) pump type, a proportioning valve is used to aspirate the desired solvent mix. between the degasser and the column. The Vanquish HPLC and UHPLC systems offer uncompromised performance, robustness, and ease of use. TP01408 RUN STOP EDIT ENTER MENU Waters 515 HPLC Pump Draw-off valve Seal wash hole (not visible) Pump head assembly High. Set a flow of 5 ml/min and purge each channel for 5 min with HPLC-quality Water. , 5-10 mL/min. Always do this one channel at a time (e. (Prime each pump head separately. This is needed to purge any air from the tubing, introduce fresh mobile phase to each line and then to VERIFY that each channel delivers the reported amount of fluid to the column (measure it). A pump influence is indicated by baseline fluctuations synchronous with the pump cycle. For modular autosamplers purge from front panel. If only an occasional bubble is present, the flow rate will be erratic, causing retention time problems. Overlay of 6 pump proportioning chromatograms in 10% gradient. Open the purge valve and set the flow rate of 5 mL/min for each channel with HPLC grade water. Before the actual compression process begins (see Fig. Prepare the Sampler • Avoid using needle wash and seat backflush with organic solvents with buffer applications, Most solvents need to be degassed, so U/HPLC systems typically include a solvent degassing system. ), prime system if necessary. 998. Priming the 1525 Binary HPLC Pump Using Empower When to Prime Priming and purging is necessary to ensure proper pump operation when: • You start the pump for the first time • You change eluent • The system has been idle for some time Parts Required None Tools/Materials Required • Priming syringe (in Startup Kit; WAT010337) Procedure 1. Flush the system. The Vanquish HPLC and UHPLC systems offer uncompromised performance, robustness, and ease of use. HPLC Pump – provides solvent flow and proportioning. After purging is done click [Pump on] button / to deliver the solvent for at least 20 to 30min to rinse the flow line . 6. Flow Connections of the Binary Pump with Solvent Selection Valve 24 Flow Connections of the Binary Pump without Solvent Selection Valve 27 Priming and Purging the System 30 2 Optimizing Performance How to optimize the binary pump to achieve best chromato-graphic results 33 Hints for Successful Use of the Binary Pump 34 Solvent. TapIn this technical article you will learn about the procedure for purging air from a reversed-phase column. 5 ml/min for 4 min, I only collected 1 ml of liquid. • Flush the entire system flow path with water for at least 10 min at 2 mL/min.